Protection of DNA During Preparative Agarose Gel Electrophoresis Against Damage Induced by Ultraviolet Light

Protection of DNA During Preparative Agarose Gel Electrophoresis Against Damage Induced by Ultraviolet Light

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Preparative gel electrophoresis of double- stranded DNA Irrigation Sleeves usually includes staining the gel with ethidium bromide followed by illumination with ultraviolet (UV-B) light.In this report, DNA isolated from agarose gels was found to be a poor substrate for in vitro transcription, transformation of E.coli and PCR.

Inhibition was not caused by enzyme- inhibiting impurities in the agarose gel, but was induced Jeans by a standard transilluminator fitted with 312-nm tubes.Interestingly, it was possible to protect the DNA against UV damage by the addition of cytidine or guanosine to the electrophoresis buffer.